在夏威夷特有植食性盲蝽（Nesosydne chambersi：Delphacidae）中分离和表征微卫星标记。

Isolation and characterization of microsatellite markers in an endemic Hawaiian planthopper (Nesosydne chambersi: Delphacidae).

机构信息

Department of Environmental Science, Policy and Management, 137 Mulford Hall, University of California, Berkeley, Berkeley, CA 94720-3114, USA.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1436-8. doi: 10.1111/j.1755-0998.2008.02253.x. Epub 2008 Sep 3.

DOI:10.1111/j.1755-0998.2008.02253.x

PMID:21586068

Abstract

We have isolated and characterized 17 microsatellite loci for the endemic Hawaiian planthopper Nesosydne chambersi (Delphacidae), a member of a large Hawaiian Nesosydne radiation. Thirty individuals from one population and 10 individuals from two populations across the species' range were tested to investigate polymorphism. The observed loci contained two to nine alleles per locus. Expected heterozygosity within this species ranged from 0.2 to 0.85. These markers will be used to assess intraspecific differentiation and population structure within N. chambersi.

摘要

我们已经分离并鉴定了 17 个微卫星位点，用于研究地方性夏威夷飞虱 Nesosydne chambersi（叶蝉科），这是夏威夷 Nesosydne 辐射的一个成员。从一个种群的 30 个个体和两个种群的 10 个个体中进行了测试，以研究多态性。观察到的位点每个位点包含 2 到 9 个等位基因。该物种内的预期杂合度范围为 0.2 到 0.85。这些标记将用于评估 N. chambersi 内的种内分化和种群结构。