School of Natural Resource Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Qld 4001, Australia.
Mol Ecol Resour. 2009 Jan;9(1):412-4. doi: 10.1111/j.1755-0998.2008.02464.x.
Fifteen microsatellite loci were isolated for population genetic studies of mulloway (Argyrosomus japonicus), a commercial/recreational fish species in Southern Australia. A genomic library was screened randomly for di- tri- and tetranucleotide repeats. Fifteen microsatellite marker loci were developed that were highly polymorphic (allele number ranged from four to 18). Observed and expected heterozygosities ranged from 0.17 to 1 and from 0.27 to 0.91, respectively. These markers have proven useful for estimating genetic variation and for evaluating population structure across the species' natural distribution. They also provide powerful tools for optimizing hatchery practices to conserve genetic diversity.
15 个微卫星位点被分离出来，用于对澳大利亚南部商业/娱乐性鱼类褐牙鲆（Argyrosomus japonicus）的群体遗传研究。通过随机筛选二核苷酸、三核苷酸和四核苷酸重复序列，构建了基因组文库。开发出的 15 个微卫星标记位点具有高度多态性（等位基因数从 4 到 18 不等）。观察到的和预期的杂合度分别在 0.17 到 1 和 0.27 到 0.91 之间。这些标记物已被证明可用于评估物种自然分布范围内的遗传变异和种群结构，同时也为优化养殖场实践以保护遗传多样性提供了有力工具。
