在红雪蟹（Chionoecetes japonicus）中分离和特征化多态性微卫星标记。

Isolation and characterization of polymorphic microsatellite markers in the red snow crab (Chionoecetes japonicus).

机构信息

Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate-shi, Hokkaido 041-8611, Japan.

出版信息

Mol Ecol Resour. 2008 May;8(3):605-7. doi: 10.1111/j.1471-8286.2007.02012.x.

DOI:10.1111/j.1471-8286.2007.02012.x

Abstract

A total of 12 polymorphic microsatellite DNA loci were isolated from the red snow crab, Chionoecetes japonicus (Brachyura: Majidae), one of important fisheries resources in the Far East. The number of alleles observed at each locus ranged from two to 19, with the observed and expected heterozygosities of 0.125-0.875 and 0.156-0.949, respectively, suggesting these loci to be a useful molecular marker for population analysis in this species. Of the 12 loci, seven also were available for genotyping of the snow crab, Chionoecetes opilio, implying these loci as a useful molecular marker in the genus Chionoecetes.

摘要

共从远东地区重要渔业资源之一的日本红雪蟹（Chionoecetes japonicus）（短尾亚目：扇蟹科）中分离出 12 个多态微卫星 DNA 位点。每个位点观察到的等位基因数范围为 2 到 19，观察到的和预期的杂合度分别为 0.125-0.875 和 0.156-0.949，表明这些位点是该物种群体分析的有用分子标记。这 12 个位点中，有 7 个也可用于雪蟹（Chionoecetes opilio）的基因分型，表明这些位点是 Chionoecetes 属的有用分子标记。