Biermann U, Herbst W, Schliesser T
Institut für Hygiene und Infektionskrankheiten, Tiere der Justus-Liebig-Universität Giessen.
Berl Munch Tierarztl Wochenschr. 1990 Mar 1;103(3):88-90.
The tenacity of viruses in liquid manure of cattle was examined in a total of five samples inoculated with ECBO-virus (strain LCR-4) representing viruses without envelope and Aujeszky virus (field isolate) representing enveloped viruses. The titers were examined at regular intervals over a period of 26 weeks. On the day of inoculation each sample had a titer of 10(5) ID50/ml. After 16 weeks complete inactivation was observed in the Aujeszky virus sample stored at 20 degrees C. The Aujeszky virus sample wich was kept at 4 degrees C at 26 weeks had a titer of 10(1,75) ID50/ml. In the samples inoculated with ECBO virus after 26 weeks of inoculation a titer of 10(3) ID50/ml was found in the manure stored at 20 degrees C. No influence on the virus titers in the liquid manure samples was observed either from pH or the number of bacteria (3,4 x 10(7)-1.16 x 10(8)/ml during the examination period.
对接种了代表无包膜病毒的ECBO病毒（LCR - 4株）和代表有包膜病毒的奥耶斯基病毒（野外分离株）的总共五个样本，研究了病毒在牛粪液肥中的存活能力。在26周的时间内定期检测病毒滴度。接种当天，每个样本的滴度为10(5) ID50/ml。16周后，在20摄氏度下储存的奥耶斯基病毒样本中观察到完全灭活。在26周时保存在4摄氏度的奥耶斯基病毒样本的滴度为10(1.75) ID50/ml。接种ECBO病毒26周后，在20摄氏度储存的粪便中发现滴度为10(3) ID50/ml。在检测期间，pH值或细菌数量（3.4×10(7)-1.16×10(8)/ml）对液肥样本中的病毒滴度均未产生影响。
