Bailey J S, Siu C H
Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, Canada.
Biochim Biophys Acta. 1990 Mar 26;1033(3):267-72. doi: 10.1016/0304-4165(90)90131-f.
Two different species of cellular retinoic acid binding proteins, CRABP-I and CRABP-II, have been found in neonatal rat pups (Bailey, J. S. and Siu, C.-H. (1988) J. Biol. Chem. 263, 9326-9332). In this report, we describe a sensitive radio-ligand binding assay for CRABP in crude tissue extracts. The assay makes use of anion-exchange high-pressure liquid chromatography which effectively separates CRABP-II from CRABP-I, thus permitting the simultaneous quantitation of these two proteins. The distribution CRABP-I and CRABP-II in various neonatal and adult tissues of the rat has been examined. CRABP-I is the predominant species of CRABP and is present in high levels in the brain, skin and testis. CRABP-II is apparently unique to the skin of neonatal animals and it becomes undetectable in adult skin. Interestingly, CRABP-II is detected at a significant level only in the adrenals of adult animals, while neonatal adrenals express only CRABP-I and not CRABP-II. CRABP-I is present in higher levels in most organs at the neonatal stage than in the adult.
在新生大鼠幼崽中发现了两种不同的细胞视黄酸结合蛋白，即CRABP-I和CRABP-II（贝利，J.S.和萧，C.-H.（1988年）《生物化学杂志》263卷，9326 - 9332页）。在本报告中，我们描述了一种用于检测粗组织提取物中CRABP的灵敏放射性配体结合测定法。该测定法利用阴离子交换高压液相色谱法，可有效将CRABP-II与CRABP-I分离，从而能够同时定量这两种蛋白质。我们研究了CRABP-I和CRABP-II在大鼠各种新生和成年组织中的分布情况。CRABP-I是CRABP的主要类型，在脑、皮肤和睾丸中含量很高。CRABP-II显然是新生动物皮肤所特有的，在成年皮肤中无法检测到。有趣的是，CRABP-II仅在成年动物的肾上腺中能检测到显著水平，而新生肾上腺仅表达CRABP-I，不表达CRABP-II。在大多数器官中，CRABP-I在新生阶段的含量高于成年阶段。
