miR-27a 的下调可能抑制胃癌细胞的增殖和耐药性。
Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells.
机构信息
Institute of Digestive Disease, Subsidary Hospital, The Medical University of Ningxia Province, Yin'chuan, Ningxia Province, China.
出版信息
J Exp Clin Cancer Res. 2011 May 13;30(1):55. doi: 10.1186/1756-9966-30-55.
AIMS
Here we aimed to firstly investigate the role of miR-27a in proliferation and multidrug resistance of gastric cancer cells.
METHODS
The role of miR-27a in gastric cancer cells was detected using MTT assay, soft agar assay, flow cytometry assay, nude mice assay, real-time PCR, western blot and reporter gene assay, etc.
RESULTS
Down-regulation of miR-27a could inhibit porliferation of gastric cancer cells in vitro and in vivo. Down-regulation of miR-27a could also confer sensitivity of drugs on gastric cancer cells, and might increase accumulation and decrease releasing amount of adriamycin in gastric cancer cells. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein and the transcriptional activity of cyclin D1, and up-regulate the expression of p21.
CONCLUSIONS
MiR-27a might play important roles in porliferation and drug resistance of gastric cancer. MiR-27a might be considered as a useful target for cancer therapy.
目的
本研究旨在首先探讨 miR-27a 在胃癌细胞增殖和多药耐药中的作用。
方法
采用 MTT 检测、软琼脂克隆形成实验、流式细胞术检测、裸鼠成瘤实验、实时 PCR、Western blot 和报告基因实验等方法检测 miR-27a 在胃癌细胞中的作用。
结果
下调 miR-27a 可抑制胃癌细胞在体外和体内的增殖。下调 miR-27a 还可以使胃癌细胞对药物敏感，并可能增加阿霉素在胃癌细胞中的蓄积，减少其释放量。下调 miR-27a 可显著降低 P-糖蛋白的表达和 cyclin D1 的转录活性，并上调 p21 的表达。
结论
miR-27a 可能在胃癌的增殖和耐药中发挥重要作用。miR-27a 可能是癌症治疗的一个有用靶点。
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