Institute of Parasitology, McGill University, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, Quebec H9X3V9, Canada.
Vet Parasitol. 2011 Sep 27;181(2-4):388-92. doi: 10.1016/j.vetpar.2011.04.012. Epub 2011 Apr 16.
Microfilariae were isolated from a Katrina rescue dog that remained microfilariaemic despite successful adulticidal treatments and repeated treatment with high doses of macrocyclic lactones (MLs). The microfilariae were genotyped at two P-glycoprotein single nucleotide polymorphic sites which had been found to correlate with reduced sensitivity to MLs. The genetic polymorphism (GG-GG), previously found to be associated with insensitivity to MLs in vitro, was present at a frequency of 45.3% in microfilariae that survived repeated treatments with high doses of ML anthelmintics. The data show phenotypic and genotypic evidence of ML resistance in Dirofilaria immitis.
从一只卡特里娜飓风救援犬中分离出微丝蚴，尽管对成虫进行了成功的杀灭处理，并反复使用大剂量大环内酯类药物（MLs）进行治疗，但该犬仍持续存在微丝蚴血症。对微丝蚴进行了两种 P 糖蛋白单核苷酸多态性位点的基因分型，这些位点与对 MLs 的敏感性降低相关。先前在体外发现与 MLs 不敏感相关的遗传多态性（GG-GG）在多次接受大剂量 ML 驱虫药物治疗后存活的微丝蚴中的频率为 45.3%。这些数据表明犬恶丝虫存在对 ML 耐药的表型和基因型证据。
