Lau Daisy Ching-Wai, McLeod Stephanie, Collaery Sara, Peou Selina, Tran Andy Truc, Liang Michelle, Šlapeta Jan
Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, NSW, 2006, Australia.
Curr Res Parasitol Vector Borne Dis. 2021 Jan 13;1:100007. doi: 10.1016/j.crpvbd.2021.100007. eCollection 2021.
For the past 30 years, chemoprophylaxis with macrocyclic lactone (ML) anthelmintics has been the primary strategy for canine heartworm () control in both the USA and Australia. ML-resistant isolates have been confirmed to exist in the USA and studies have shown that 42 single nucleotide polymorphisms (SNPs) are associated with phenotypic ML-resistance. Currently, ML-resistance has not been reported in any Australian clinical cases of canine heartworm. The aim of the study is to determine whether the 42 SNPs associated with resistance to MLs in the isolates from the USA are present in adult heartworms from a clinical case in Australia. Five adult obtained from a dog at post-mortem (Sydney, Australia) were sequenced using the Illumina sequencing technology. The genomic analyses revealed 6 out of the 42 SNPs associated with ML-resistance to be present in our samples, 3 out of the 6 SNPs identified were nonsynonymous SNPs but not in candidate genes for ML-resistance. ML-susceptibility profile was mixed using the 42-SNP and 10-SNP models, but the 5-SNP, 3-SNP and 2-SNP models demonstrated ML susceptibility for all five individuals. In this study, the first whole-genome reference of from Australia establishes a new baseline for comparative studies and will be valuable for tracking ML-resistance emergence.
在过去30年里，在美国和澳大利亚，使用大环内酯（ML）驱虫药进行化学预防一直是犬心丝虫控制的主要策略。在美国已证实存在对ML耐药的分离株，研究表明42个单核苷酸多态性（SNP）与ML耐药表型相关。目前，在澳大利亚任何犬心丝虫临床病例中均未报告ML耐药情况。本研究的目的是确定在美国分离株中与ML耐药相关的42个SNP是否存在于澳大利亚一例临床病例的成年心丝虫中。使用Illumina测序技术对从一只狗尸检（澳大利亚悉尼）获得的5条成年心丝虫进行了测序。基因组分析显示，我们的样本中存在42个与ML耐药相关的SNP中的6个，所鉴定的6个SNP中有3个是非同义SNP，但不在ML耐药候选基因中。使用42-SNP和10-SNP模型时ML易感性情况不一，但5-SNP、3-SNP和2-SNP模型显示所有5个个体均对ML敏感。在本研究中，澳大利亚心丝虫的首个全基因组参考为比较研究建立了新的基线，对于追踪ML耐药的出现将具有重要价值。
