甲状腺髓样癌和小细胞肺癌培养细胞系中降钙素基因调控DNA序列的差异利用
Differential utilization of calcitonin gene regulatory DNA sequences in cultured lines of medullary thyroid carcinoma and small-cell lung carcinoma.
作者信息
de Bustros A, Lee R Y, Compton D, Tsong T Y, Baylin S B, Nelkin B D
机构信息
School of Medicine, Johns Hopkins University, Baltimore, Maryland 21231.
出版信息
Mol Cell Biol. 1990 Apr;10(4):1773-8. doi: 10.1128/mcb.10.4.1773-1778.1990.
Regulation of expression of the human calcitonin gene was found to differ between two tumor lines of different tissue origin, medullary thyroid carcinoma (TT line) and small-cell lung carcinoma (DMS53 line). Distal 5' DNA elements between -750 and -2000 exhibited a stronger basal activity in DMS53 than in TT cells, whereas proximal DNA sequences between -132 and -252 mediated a dramatic cyclic AMP response in TT but not DMS53 cells.
研究发现，人降钙素基因的表达调控在两种不同组织来源的肿瘤细胞系中存在差异，即甲状腺髓样癌（TT细胞系）和小细胞肺癌（DMS53细胞系）。-750至-2000之间的远端5' DNA元件在DMS53细胞中的基础活性比在TT细胞中更强，而-132至-252之间的近端DNA序列在TT细胞中可介导显著的环磷酸腺苷反应，在DMS53细胞中则不然。
Zotero 插件