Samra Z, Rosenberg S, Dan M
Chlamydia and Mycoplasma National Center, Department of Microbiology, Rabin Medical Center, Beillinson Campus, Petah Tikva, Israel.
J Chemother. 2011 Apr;23(2):77-9. doi: 10.1179/joc.2011.23.2.77.
The in vitro activity of tetracycline, doxycycline, erythromycin, roxithromycin, clarithromycin, azithromycin, levofloxacin and moxifloxacin was tested against 63 clinical isolates of Ureaplasma urealyticum. The minimal inhibitory concentrations (MICs) and the minimal bactericidal concentrations (MBCs) were determined by the broth microdilution method in A7 medium. The MIC(50) and MIC(90) of the tested agents after 24 h of incubation were as follows: tetracycline, 0.5 and 2.0 μg/ml; doxycycline, 0.125 and 0.25 μg/ml; erythromycin, 2.0 and 8.0 μg/ml; roxithromycin, 2.0 and 4.0 μg/ml; clarithromycin, 0.25 and 1.0 μg/ml; azithromycin, 2.0 and 4.0 μg/ml; levofloxacin, 1.0 and 2.0 μg/ml; and moxifloxacin, 0.5 and 0.5 μg/ml, respectively. The MIC values after 24 h and 48 h incubation differed by no more than one dilution for all the agents with the exception of doxycycline (two dilution difference for MIC(90)). Overall, moxifloxacin was the most active agent in vitro against U. urealyticum, with the narrowest difference between MIC and MBC values, followed closely by levofloxacin. Clarithromycin was the most active macrolide.
测试了四环素、多西环素、红霉素、罗红霉素、克拉霉素、阿奇霉素、左氧氟沙星和莫西沙星对63株解脲脲原体临床分离株的体外活性。采用肉汤微量稀释法在A7培养基中测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。孵育24小时后，受试药物的MIC50和MIC90如下：四环素，0.5和2.0μg/ml；多西环素，0.125和0.25μg/ml；红霉素，2.0和8.0μg/ml；罗红霉素，2.0和4.0μg/ml；克拉霉素，0.25和1.0μg/ml；阿奇霉素，2.0和4.0μg/ml；左氧氟沙星，1.0和2.0μg/ml；莫西沙星，0.5和0.5μg/ml。除多西环素外（MIC90有两个稀释度差异），所有药物孵育24小时和48小时后的MIC值差异不超过一个稀释度。总体而言，莫西沙星是体外抗解脲脲原体活性最强的药物，MIC和MBC值之间的差异最小，其次是左氧氟沙星。克拉霉素是活性最强的大环内酯类药物。
