Max Planck Institute of Molecular Plant Physiology, Department of Organelle Biology, Biotechnology and Molecular Ecophysiology, Energy Metabolism Research Group, D-14476 Potsdam-Golm, Germany.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):822-826. doi: 10.1099/ijs.0.026955-0. Epub 2011 May 13.
Bacterial strain G72T was isolated from surface-sterilized roots of Arabidopsis thaliana growing in its natural habitat. This Gram-positive, rod-shaped, non-motile, microaerophilic and aerobically growing isolate was characterized by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, strain G72T belongs to the genus Microbacterium. However, reassociation values in a DNA-DNA hybridization analysis with closely related strains were between 45.1 and 15.9 %. The DNA G+C content was 70.1 mol%. Strain G72T possessed a peptidoglycan type based on B2β with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg-Gly-D-Orn). The isolate contained MK-13, MK-12 and MK-11 as major respiratory quinones and anteiso-C15:0, anteiso-C17:0 and iso-C16:0 as predominant fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids. Based on its physiological and biochemical traits, as well as genotypic results, strain G72T is considered to represent a novel species, for which the name Microbacterium yannicii sp. nov. is proposed. The type strain is G72T (=DSM 23203T=LMG 25521T).
细菌菌株 G72T 是从生长在其自然栖息地的拟南芥表面消毒的根部中分离出来的。这种革兰氏阳性、杆状、非运动、微需氧和需氧生长的分离株是通过多相方法进行表征的。根据 16S rRNA 基因序列相似性，菌株 G72T 属于微杆菌属。然而，与密切相关的菌株进行 DNA-DNA 杂交分析的重配值在 45.1%和 15.9%之间。DNA G+C 含量为 70.1 mol%。菌株 G72T 具有基于 B2β的肽聚糖类型，其中部分谷氨酸被 3-羟基谷氨酸（Glu/Hyg-Gly-D-Orn）取代。该分离株含有 MK-13、MK-12 和 MK-11 作为主要呼吸醌和 anteiso-C15:0、anteiso-C17:0 和 iso-C16:0 作为主要脂肪酸。主要极性脂质是双磷脂酰甘油、磷脂酰甘油和三种未鉴定的糖脂。根据其生理生化特征以及基因型结果，菌株 G72T 被认为代表一种新的物种，因此提议使用 Microbacterium yannicii sp. nov. 这个名称。模式菌株是 G72T（=DSM 23203T=LMG 25521T）。
