Axenov-Gribanov Denis V, Kostka Daria V, Vasilieva Ulyana А, Shatilina Zhanna M, Krasnova Maria E, Pereliaeva Ekaterina V, Zolotovskaya Elena D, Morgunova Maria M, Rusanovskaya Olga O, Timofeyev Maxim A
Irkutsk State University, Karl Marx St. 1, 664003 Irkutsk, Russia.
Irkutsk Regional Clinical Advisory and Diagnostic Center, Baykalskaya Str., 109 Irkutsk, Russia.
Int J Microbiol. 2020 Jul 27;2020:5359816. doi: 10.1155/2020/5359816. eCollection 2020.
Inadequate use of antibiotics has led to spread of microorganisms resistant to effective antimicrobial compounds for humans and animals. This study was aimed to isolate cultivable strains of actinobacteria associated with Baikal endemic alga and estimate their antibiotic properties. During this study, we isolated both widespread and dominant strains related to the genus and representatives of the genera , and . For the first time, actinobacteria belonging to the genera and were isolated from Baikal ecosystem. Also, it was the first time when actinobacteria of the genus were isolated from freshwater algae. Some rare strains demonstrated activity inhibiting growth of bacteria and yeasts. Also, it has been shown that the strains associated with Baikal alga are active against both Gram-positive and Gram-negative bacteria. According to this study and previously published materials, diversity of cultivable actinobacteria and rare strains isolated from is comparable to that of cultivable actinobacteria previously isolated from plant sources of Lake Baikal. Also, it exceeds the cultivable actinobacteria diversity previously described for macroinvertebrates, water, or sediments of Lake Baikal. The large number of rare and active strains associated with the endemic alga could be the promising sources for biopharmaceutical and biotechnological developments and discovery of new natural compounds.
抗生素使用不当导致对人类和动物有效的抗菌化合物产生抗性的微生物传播。本研究旨在分离与贝加尔湖特有藻类相关的可培养放线菌菌株，并评估它们的抗生素特性。在这项研究中，我们分离出了与该属相关的广泛分布和优势菌株以及、和属的代表菌株。首次从贝加尔湖生态系统中分离出属于和属的放线菌。此外，这也是首次从淡水藻类中分离出属的放线菌。一些稀有菌株表现出抑制细菌和酵母生长的活性。此外，研究表明，与贝加尔湖藻类相关联的菌株对革兰氏阳性菌和革兰氏阴性菌均有活性。根据本研究及先前发表的资料，从分离出的可培养放线菌和稀有菌株的多样性与先前从贝加尔湖植物来源分离出的可培养放线菌的多样性相当。此外，它超过了先前描述的贝加尔湖大型无脊椎动物、水或沉积物中的可培养放线菌多样性。与特有藻类相关的大量稀有和活性菌株可能是生物制药和生物技术发展以及发现新天然化合物的有前途的来源。
