Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):786-790. doi: 10.1099/ijs.0.028951-0. Epub 2011 May 13.
A Gram-positive, strictly aerobic, rod-shaped, non-motile bacterial strain, designated MJ28T, was isolated from a sludge sample from the Daejeon sewage disposal plant in South Korea. A polyphasic approach was applied to study the taxonomic position of strain MJ28T. Strain MJ28T showed highest 16S rRNA gene sequence similarity to Pseudoclavibacter soli KP02T (95.2 %). Levels of 16S rRNA gene sequence similarity to the type strains of other Pseudoclavibacter species were less than 94.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ28T belonged to the clade formed by members of the genus Pseudoclavibacter in the family Microbacteriaceae. The G+C content of the genomic DNA of strain MJ28T was 65.8 mol%. The chemotaxonomic characteristics of strain MJ28T showed features typical of the genus Pseudoclavibacter, with MK-9 as the predominant respiratory quinone, 2,4-diaminobutryic acid as the diamino acid in the peptidoglycan, and anteiso-C17:0 (44.6 %), anteiso-C15:0 (35.7 %) and C16:0 (9.5 %) as the major fatty acids. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ28T is considered to represent a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter caeni sp. nov. is proposed. The type strain is MJ28T (=KCTC 19773T=JCM 16921T).
一株革兰氏阳性、严格需氧、杆状、非运动的细菌菌株，被命名为 MJ28T，从韩国大田污水处理厂的污泥样本中分离得到。应用多相分类法研究菌株 MJ28T 的分类地位。菌株 MJ28T 与土壤杆菌属的假诺卡氏菌 KP02T 的 16S rRNA 基因序列相似性最高，为 95.2%。与其他假诺卡氏菌属的模式菌株的 16S rRNA 基因序列相似性均低于 94.0%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MJ28T 属于微杆菌科土壤杆菌属的分支。菌株 MJ28T 的基因组 DNA G+C 含量为 65.8mol%。菌株 MJ28T 的化学分类特征表现出典型的假诺卡氏菌属特征，主要呼吸醌为 MK-9，肽聚糖中的二氨基庚二酸为 2,4-二氨基丁酸，主要脂肪酸为anteiso-C17:0（44.6%）、anteiso-C15:0（35.7%）和 C16:0（9.5%）。基于系统发育推断、脂肪酸图谱和其他表型特性，菌株 MJ28T 被认为代表假诺卡氏菌属的一个新种，命名为假诺卡氏菌土壤亚种。其模式菌株为 MJ28T（=KCTC 19773T=JCM 16921T）。
