Research and Development Division, H-Plus Eco Ltd, BVC #301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1265-1270. doi: 10.1099/ijs.0.033316-0. Epub 2011 Jul 15.
A Gram-stain-positive, strictly aerobic, spherical, non-motile red-pigmented bacterial strain, designated MJ27(T), was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was used to study the taxonomic position of strain MJ27(T). Strain MJ27(T) shared highest 16S rRNA gene sequence similarity with Deinococcus grandis DSM 3963(T) (98.8 %), Deinococcus caeni Ho-08(T) (97.5 %) and Deinococcus aquaticus PB314(T) (96.6 %.); levels of sequence similarity with the type strains of other Deinococcus species were less than 96.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ27(T) belonged to the clade formed by members of the genus Deinococcus in the family Deinococcaceae. The G+C content of the genomic DNA of strain MJ27(T) was 67.6 mol%. The chemotaxonomic characteristics of strain MJ27(T) were typical of members of the genus Deinococcus, with MK-8 as the predominant respiratory quinone, C(16:1)ω7c, C(15:1)ω6c, C(16:0) and C(15:0) as major fatty acids (>12 %), ornithine as the diamino acid in the cell-wall peptidoglycan and resistance to gamma radiation [D(10) (dose required to reduce the bacterial population by tenfold) >9 kGy]. The low levels of DNA-DNA relatedness reported here (5.3±1.5-29.2±2.3 %) indicate that strain MJ27(T) represents a species that is separate from its closest relatives in the genus Deinococcus. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ27(T) is considered to represent a novel species of the genus Deinococcus, for which the name Deinococcus daejeonensis sp. nov. is proposed. The type strain is MJ27(T) ( = KCTC 13751(T) = JCM 16918(T)).
一株革兰氏阳性、严格需氧、球形、非运动性、红色素产生的细菌菌株，被命名为 MJ27(T)，从韩国大田污水处理厂的污泥样本中分离得到。采用多相研究方法来研究菌株 MJ27(T)的分类地位。菌株 MJ27(T)与 Deinococcus grandis DSM 3963(T)（98.8%）、Deinococcus caeni Ho-08(T)（97.5%）和 Deinococcus aquaticus PB314(T)（96.6%）的 16S rRNA 基因序列相似性最高；与其他 Deinococcus 种的模式菌株的序列相似性均小于 96.0%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MJ27(T)属于 Deinococcaceae 家族的 Deinococcus 属中的一个分支。菌株 MJ27(T)的基因组 DNA G+C 含量为 67.6mol%。菌株 MJ27(T)的化学分类特征是典型的 Deinococcus 属成员，主要呼吸醌为 MK-8，主要脂肪酸为 C(16:1)ω7c、C(15:1)ω6c、C(16:0)和 C(15:0)（>12%），细胞壁肽聚糖中的二氨基氨基酸为鸟氨酸，对γ辐射有抗性[D(10)（使细菌种群减少十倍所需的剂量）>9 kGy]。这里报道的低 DNA-DNA 同源性（5.3±1.5-29.2±2.3%）表明，菌株 MJ27(T)代表了与 Deinococcus 属中最接近的亲缘关系的一个分离物种。基于系统发育推断、脂肪酸图谱和其他表型特性，菌株 MJ27(T)被认为是 Deinococcus 属的一个新种，建议将其命名为 Deinococcus daejeonensis sp. nov.。模式菌株为 MJ27(T)（=KCTC 13751(T)=JCM 16918(T)）。
