Fushida S, Yonemura Y, Urano T, Yamaguchi A, Miyazaki I, Nakamura T, Shiku H
KANAZAWA UNIV,SCH MED,DEPT SURG 2,13-1 TAKARAMACHI,KANAZAWA,ISHIKAWA 920,JAPAN. NAGASAKI UNIV,SCH MED,DEPT ONCOL,NAGASAKI 852,JAPAN. KYUSHU UNIV,SCH MED,DEPT BIOL,HIGASHI KU,FUKUOKA 812,JAPAN.
Int J Oncol. 1993 Dec;3(6):1067-70. doi: 10.3892/ijo.3.6.1067.
It has become evident that the hepatocyte growth factor (HGF) could be involved in the growth of various epithelial cells. In addition, it has recently been elucidated that the c-met gene, a proto-oncogene, encodes the HGF receptor. We examined the expression of HGF and c-met gene in human gastric cancer, using seven gastric cancer cell lines. The Northern blotting method revealed that, among seven cell lines used, only MKN45 expressed the c-met gene at a high efficiency, while the Southern blotting method demonstrated the amplification of the c-met gene. When the expression of HGF gene was studied by the Northern blot method, the expression of this gene was also observed only in MKN45, which was confirmed by immunostaining with polyclonal antibodies to HGF. The above results suggest the possibility that in MKN45, a gastric cancer cell line, the autocrine mechanism by HGF-met might be involved in its growth.
现已明确，肝细胞生长因子（HGF）可能参与多种上皮细胞的生长。此外，最近已阐明原癌基因c-met基因编码HGF受体。我们使用七种胃癌细胞系研究了HGF和c-met基因在人胃癌中的表达。Northern印迹法显示，在所使用的七种细胞系中，只有MKN45高效表达c-met基因，而Southern印迹法证明了c-met基因的扩增。当通过Northern印迹法研究HGF基因的表达时，也仅在MKN45中观察到该基因的表达，这通过用抗HGF多克隆抗体进行免疫染色得到证实。上述结果提示，在胃癌细胞系MKN45中，HGF-met的自分泌机制可能参与其生长。
