Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium.
Int J Oncol. 2011 Jul;39(1):51-9. doi: 10.3892/ijo.2011.1031. Epub 2011 May 4.
The aim of this study was to determine the prevalence of human papillomavirus (HPV) in patients with laryngeal benign lesions (LBLs) and laryngeal squamous cell carcinomas (LSCCs) using a sensitive E6/E7 type-specific PCR. Paraffin-embedded samples from LBL (n=39) and LSCC patients (n=67) were evaluated for the presence of HPV DNA by GP5+/GP6+ consensus PCR and E6/E7 type-specific PCR for HPV types 6, 11, 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68. In LSCCs, immunohistochemical staining of p16, p53 and EGFR was also assessed. The E6/E7 type-specific PCR showed that 44 out of 59 LSCC patients (i.e., 75%) had high-risk (hr) HPV types and that 27 out of 35 LBL patients (i.e., 77%) had hrHPV types. HPV-16 viral load was significantly higher in LSCC than in LBL patients (p<10-6). The presence of hrHPV DNA did not correlate with the proportion of disease-free patients. Comparable levels of p16, p53 and EGFR expression were observed in the hrHPV+ tumor group (100% p16+, 56% p53+ and 97% EGFR+) and in the HPV- or low-risk (lr) HPV+ tumor group (92% p16+, 66% p53+ and 100% EGFR+). A very high prevalence of oncogenic HPV-16 was found in a series of benign and malignant laryngeal lesions. LSCC appears to be characterized by an active hrHPV infection. In LSCCs, the hrHPV+ subgroup had a similar prognosis (in terms of risk of recurrence) as the HPV- subgroup.
本研究旨在利用敏感的 E6/E7 型特异性 PCR 检测喉良性病变（LBL）和喉鳞状细胞癌（LSCC）患者中人类乳头瘤病毒（HPV）的流行率。通过 GP5+/GP6+ 共识 PCR 和 HPV 型 6、11、16、18、31、33、35、39、45、51、52、53、56、58、59、66 和 68 的 E6/E7 型特异性 PCR 评估 LBL（n=39）和 LSCC 患者（n=67）石蜡包埋样本中 HPV DNA 的存在。在 LSCC 中，还评估了 p16、p53 和 EGFR 的免疫组织化学染色。E6/E7 型特异性 PCR 显示 59 例 LSCC 患者中有 44 例（即 75%）有高危（hr）HPV 型，35 例 LBL 患者中有 27 例（即 77%）有 hrHPV 型。LSCC 患者的 HPV-16 病毒载量明显高于 LBL 患者（p<10-6）。hrHPV DNA 的存在与无病患者的比例无关。在 hrHPV+肿瘤组（100% p16+、56% p53+和 97% EGFR+）和 HPV-或低危（lr）HPV+肿瘤组（92% p16+、66% p53+和 100% EGFR+）中观察到相似水平的 p16、p53 和 EGFR 表达。在一系列良性和恶性喉病变中发现了致癌 HPV-16 的非常高流行率。LSCC 似乎以活跃的 hrHPV 感染为特征。在 LSCC 中，hrHPV+亚组的复发风险（即复发风险）与 HPV-亚组相似。
