miR-185 和 miR-133b 的失调与结直肠癌的总生存期和转移有关。

miR-185 and miR-133b deregulation is associated with overall survival and metastasis in colorectal cancer.

机构信息

Department of Molecular Medicine and Surgery, CMM L8:01, Karolinska University Hospital-Solna, SE-17176 Stockholm, Sweden.

出版信息

Int J Oncol. 2011 Aug;39(2):311-8. doi: 10.3892/ijo.2011.1043. Epub 2011 May 13.

DOI:10.3892/ijo.2011.1043

PMID:21573504

Abstract

Colorectal cancer (CRC) is one of the most common and deadly forms of cancer. Despite improved treatment modalities, post-operative recurrence and metastasis remain the major problems for extending patient survival after surgery. This highlights the need to search for biomarkers for prognostication and treatment stratification of colorectal cancer patients. In this study, we applied the SYBR-green quantitative PCR-based array approach to screen for differentially expressed miRNAs between patients with short (<50 months, range 10-33 months) and long survival (≥ 50 months, range 50-152 months). The selected candidate prognostic miRNAs were validated in a cohort of 50 CRC patients by TaqMan quantitative PCR. We found that high expression of miR-185 and low expression of miR-133b were correlated with poor survival (p=0.001 and 0.028, respectively) and metastasis (p=0.007 and 0.036, respectively) in colorectal cancer. Our findings suggest the potential prognostic values of these miRNAs for predicting clinical outcome after surgery.

摘要

结直肠癌（CRC）是最常见和最致命的癌症之一。尽管治疗方式有所改善，但手术后的复发和转移仍然是延长患者生存的主要问题。这凸显了寻找生物标志物用于预测结直肠癌患者预后和治疗分层的必要性。在这项研究中，我们应用基于 SYBR-green 定量 PCR 的阵列方法筛选短生存（<50 个月，范围 10-33 个月）和长生存（≥50 个月，范围 50-152 个月）患者之间差异表达的 miRNAs。通过 TaqMan 定量 PCR 在 50 例 CRC 患者的队列中验证了选定的候选预后 miRNAs。我们发现 miR-185 高表达和 miR-133b 低表达与结直肠癌患者的不良生存（p=0.001 和 0.028）和转移（p=0.007 和 0.036）相关。我们的研究结果表明，这些 miRNAs 具有预测手术后临床结局的潜在预后价值。