Servicio de Obstetricia, Hospital Universitario y Politécnico La Fe, Valencia, Spain.
Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, Valencia, Spain.
Epigenetics. 2022 Nov;17(11):1345-1356. doi: 10.1080/15592294.2021.2023271. Epub 2021 Dec 30.
To compare the expression of microRNA-185-5p (miR-185-5p) in normal foetuses and in foetuses with late-onset growth restriction (FGR) and to determine the factors influencing this expression. In a prospective study, 40 foetuses (22 of them with late-onset FGR and 18 with normal growth) were scanned with Doppler ultrasound after week 35 and followed until birth. Subsequently, blood samples from umbilical cords were collected after delivery to evaluate the expression of miR-185-5p using real-time qPCR. Finally, multivariable regression analysis was applied to determine the clinical and ultrasonographic factors influencing miR-185-5p expression in both normal and late-onset FGR foetuses. In comparison with normal foetuses, late-onset FGR foetuses expressed upregulation of miR-185-5p (2.26 ± 1.30 versus 1.27 ± 1.03 2^-ddCt, P = 0.011). Multivariable regression analysis confirmed that cerebroplacental ratio (P < 0.05) was the only determinant of this overexpression. FGR foetuses overexpress miR-185-5p in relation to brain-sparing. Future studies will be needed to investigate the role of miR-185 in the management of late-onset FGR.
比较正常胎儿和晚发型生长受限（FGR）胎儿中微小 RNA-185-5p（miR-185-5p）的表达，并确定影响其表达的因素。在一项前瞻性研究中，对 35 周后进行多普勒超声扫描的 40 名胎儿（其中 22 名为晚发型 FGR，18 名为正常生长）进行了研究，并随访至分娩。随后，在分娩后采集脐带血样，通过实时 qPCR 评估 miR-185-5p 的表达。最后，应用多变量回归分析确定影响正常和晚发型 FGR 胎儿 miR-185-5p 表达的临床和超声因素。与正常胎儿相比，晚发型 FGR 胎儿 miR-185-5p 的表达上调（2.26 ± 1.30 与 1.27 ± 1.03 2^-ddCt，P = 0.011）。多变量回归分析证实，脑胎盘比（P < 0.05）是这种过表达的唯一决定因素。与脑保护相关，FGR 胎儿过度表达 miR-185-5p。未来的研究将需要调查 miR-185 在晚发型 FGR 管理中的作用。
