Jameel A, Law M, Coombes R, Luqmani Y
CHARING CROSS HOSP,DEPT MED ONCOL,FULHAM PALACE RD,LONDON W6 8RF,ENGLAND. ROYAL CANC HOSP,INST CANC RES,EPIDEMIOL SECT,SUTTON SM2 5NG,SURREY,ENGLAND.
Int J Oncol. 1993 Jun;2(6):1075-80. doi: 10.3892/ijo.2.6.1075.
A human breast cancer cDNA library was screened with a polyclonal antiserum against breast cancer metastasis membranes in order to select genes whose expression may be altered in breast malignancy. Several immunopositive clones have been isolated. One of these, AJ1, was analyzed in detail and found to be expressed at varying levels as a 3.3 kb mRNA in all of the 123 primary breast cancers examined. AJ1 was expressed at lower levels in non-malignant biopsies as compared to malignant tissue (p=0.0008). We found high expression to be associated with lymph node involvement (p=0.059). Comparison between high and low expressing groups showed a significant difference for both overall (p=0.039) and relapse-free (p=0.034) survival at 10 years, with early recurrence and reduced overall survival in high expressors. Thus, AJ1 could be a potentially useful prognostic factor and marker of aggressiveness in breast cancer. Partial sequence analysis of the 620 bp clone showed complete homology with human heat shock protein, HSP 90.
用人乳腺癌转移膜多克隆抗血清筛选人乳腺癌cDNA文库，以选择在乳腺恶性肿瘤中表达可能改变的基因。已分离出几个免疫阳性克隆。其中一个名为AJ1的克隆进行了详细分析，发现在所检测的123例原发性乳腺癌中，其作为3.3 kb mRNA以不同水平表达。与恶性组织相比，AJ1在非恶性活检组织中的表达水平较低（p = 0.0008）。我们发现高表达与淋巴结受累相关（p = 0.059）。高表达组和低表达组之间的比较显示，10年总生存率（p = 0.039）和无复发生存率（p = 0.034）均有显著差异，高表达者早期复发且总生存率降低。因此，AJ1可能是一种潜在有用的乳腺癌预后因素和侵袭性标志物。对620 bp克隆的部分序列分析显示与人热休克蛋白HSP 90完全同源。
