Kato S, Morita T, Takenaka T, Kato M, Hirano A, Herz F, Ohama E
Division of Neuropathology, Faculty of Medicine, Tottori University, Yonago, Japan.
Acta Neuropathol. 1995;89(2):184-8. doi: 10.1007/BF00296364.
This retrospective study deals with the expression of stress-response (heat-shock) protein 90 (srp 90) in a series of 148 human brain tumors. Immunohistochemical procedures were employed; cells of the human breast cancer line MCF7 exposed to hyperosmolar stress served as positive controls. Deposits of reaction products were found in the cytoplasm and they displayed a granular pattern. srp 90 was detected in 14/31 meningiomas and 5/10 breast cancer metastases to the brain. The protein was also present in 6/13 glioblastomas and 7/18 astrocytomas. In addition, a positive reaction was found in 2/10 medulloblastomas, 2/14 primitive neuroectodermal tumors, 1/11 pituitary tumor, 2/21 schwannomas and 2/11 lung tumor metastases; however, oligodendrogliomas and primary malignant lymphomas were not stained. The srp 90 was detected in Western blots of meningioma tissue homogenates. No significant immunohistochemical reaction was seen with sections of normal human cerebra, brain stem, cerebella, pituitary glands and spinal cords. These results document the expression of srp 90 by a variety of primary and metastatic intracranial tumors.
这项回顾性研究探讨了应激反应（热休克）蛋白90（srp 90）在148例人脑肿瘤中的表达情况。采用了免疫组织化学方法；将暴露于高渗应激的人乳腺癌细胞系MCF7的细胞用作阳性对照。在细胞质中发现了反应产物沉积，呈颗粒状。在31例脑膜瘤中的14例以及10例脑转移乳腺癌中的5例检测到srp 90。该蛋白也存在于13例胶质母细胞瘤中的6例以及18例星形细胞瘤中的7例。此外，在10例髓母细胞瘤中的2例、14例原始神经外胚层肿瘤中的2例、11例垂体瘤中的1例、21例神经鞘瘤中的2例以及11例肺转移瘤中的2例发现了阳性反应；然而，少突胶质细胞瘤和原发性恶性淋巴瘤未被染色。在脑膜瘤组织匀浆的蛋白质印迹法中检测到了srp 90。在正常人脑、脑干、小脑、垂体和脊髓切片中未观察到明显的免疫组织化学反应。这些结果证明了多种原发性和转移性颅内肿瘤中srp 90的表达。
