State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Changle Western Road, 169, Xi'an, Shaanxi Province, 710032, China.
Int J Colorectal Dis. 2011 Nov;26(11):1397-404. doi: 10.1007/s00384-011-1237-1. Epub 2011 May 15.
MGb2, a mouse-derived monoclonal antibody specific to gastric carcinoma, was developed in our laboratory. Nevertheless, the potential role of MGb2-antigen/TRAK1 (MGb2-Ag/TRAK1) in colorectal cancer (CRC) is unclear. The aim of this study was to investigate the relationship between MGb2-Ag/TRAK1 expression and the clinicopathological characteristics of CRC. The potential utility of MGb2-Ag/TRAK1 expression as a prognostic indicator was also evaluated.
Immunohistochemistry and western blot were used to detect MGb2-Ag/TRAK1 expression in 140 CRC tissues. The relationship between MGb2-Ag/TRAK1 expression and clinicopathological characteristics and postoperative survival time was statistically analyzed.
MGb2-Ag/TRAK1 expression in CRC tissues was significantly higher than in normal tissues and was positively correlated with tumor differentiation (p = 0.006), invasion (p = 0.049), and pathological stage (p = 0.032). There was no significant difference between MGb2-Ag/TRAK1 expression and the age or gender of the patient, lymphatic invasion, or distant metastasis (p = 0.586, 0.308, 0.910, and 0.068, respectively). The survival time of CRC patients with high expression of MGb2-Ag/TRAK1 was shorter than the survival time of patients with low MGb2-Ag/TRAK1 expression. Both univariate and multivariate analyses showed that tumor differentiation and MGb2-Ag/TRAK1 expression were two independent and prognostic factors for CRC (p < 0.001).
MGb2-Ag/TRAK1 may play an important role in the development of CRC and may be a valuable prognostic indicator of CRC.
我们实验室开发了一种针对胃癌的鼠源性单克隆抗体 MGb2。然而，MGb2 抗原/TRAK1（MGb2-Ag/TRAK1）在结直肠癌（CRC）中的潜在作用尚不清楚。本研究旨在探讨 MGb2-Ag/TRAK1 表达与 CRC 临床病理特征的关系，并评估 MGb2-Ag/TRAK1 表达作为预后指标的潜在效用。
采用免疫组织化学和 Western blot 检测 140 例 CRC 组织中 MGb2-Ag/TRAK1 的表达，统计分析 MGb2-Ag/TRAK1 表达与临床病理特征及术后生存时间的关系。
CRC 组织中 MGb2-Ag/TRAK1 的表达明显高于正常组织，且与肿瘤分化（p=0.006）、浸润（p=0.049）和病理分期（p=0.032）呈正相关。MGb2-Ag/TRAK1 表达与患者年龄、性别、淋巴浸润或远处转移无显著差异（p=0.586、0.308、0.910 和 0.068）。MGb2-Ag/TRAK1 高表达的 CRC 患者的生存时间短于 MGb2-Ag/TRAK1 低表达的患者。单因素和多因素分析均表明，肿瘤分化和 MGb2-Ag/TRAK1 表达是 CRC 的两个独立预后因素（p<0.001）。
MGb2-Ag/TRAK1 可能在 CRC 的发生发展中起重要作用，可能是 CRC 的一个有价值的预后指标。
