Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
Sleep Breath. 2012 Jun;16(2):505-10. doi: 10.1007/s11325-011-0532-y. Epub 2011 May 15.
The objective of this study was to investigate the effects of chronic intermittent hypoxia (CIH) on genioglossal ultrastructure and mitochondrial function as well as the intervention role of adiponectin (Ad).
Forty-two Wistar rats were randomly divided into three groups with 14 rats in each. Rats in group A were kept breathing normal air, while rats in both groups B and C received the same CIH environment (a 2-min cycle, 1 min on, 1 min off with a nadir O(2) at 4-5%, 8 h/day for successive 5 weeks). However, rats in group C was given regular intravenous Ad injection (10 μg per time, twice a week for successive 5 weeks). A simultaneous intravenous injection of saline (0.5 ml per time) was carried in groups A and B. At the end of experiment, the genioglossal ultrastructure, the serum adiponectin levels, the mitochondrial membrane potential (ΔΨ(m)), and activities of respiratory chain complexes I and IV in mitochondrion of genioglossal cells were compared among groups.
Serum Ad level was significantly lower in group B than that in group A (P < 0.01). In group B, there were genioglussal myofibril discontinuities, lysis of myofilament, edema of mitochondria, and disruption of cristae, vacuolus, and lysis of some mitochondria. These pathological changes were less significant in group C. The relative value of ΔΨ(m) was the lowest in group B but the highest in group A (P < 0.01), with group B in between. The concentrations of mitochondrial complexes I and IV in group B were the lowest but became higher and higher from group C to A, with a significant difference among groups (all P < 0.05).
CIH could lead to hypoadiponectinemia, impaired genioglossal ultrastructure, and mitochondrial dysfunction. These changes could be improved by supplement of Ad.
本研究旨在探讨慢性间歇性低氧（CIH）对颏舌肌超微结构和线粒体功能的影响，以及脂联素（Ad）的干预作用。
42 只 Wistar 大鼠随机分为 3 组，每组 14 只。A 组大鼠呼吸正常空气，B、C 组大鼠接受相同的 CIH 环境（2min 周期，1min 开，1min 关，最低 O2 为 4-5%，连续 5 周每天 8h）。然而，C 组大鼠给予常规静脉内 Ad 注射（每次 10μg，连续 5 周每周 2 次）。A、B 组同时给予生理盐水（每次 0.5ml）静脉注射。实验结束时，比较各组颏舌肌超微结构、血清脂联素水平、线粒体膜电位（ΔΨ（m））和线粒体呼吸链复合物 I 和 IV 的活性。
与 A 组相比，B 组血清 Ad 水平显著降低（P<0.01）。B 组颏舌肌肌原纤维连续性中断、肌丝溶解、线粒体水肿、嵴断裂、空泡形成，部分线粒体溶解。C 组上述病变较轻。B 组ΔΨ（m）相对值最低，A 组最高（P<0.01），B 组介于两者之间。B 组线粒体复合物 I 和 IV 浓度最低，而 C 组最高，A 组次之，组间差异有统计学意义（均 P<0.05）。
CIH 可导致低脂联素血症、颏舌肌超微结构损伤和线粒体功能障碍，补充 Ad 可改善这些变化。
