Tripathy D N, Wittek R
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Avian Dis. 1990 Jan-Mar;34(1):218-20.
A vaccinia virus promoter was evaluated for regulation of a foreign gene in fowlpox virus by a transient expression assay. Fowlpox virus-infected quail cells, transfected with plasmid DNA containing chloramphenicol acetyltransferase (CAT) gene ligated to a vaccinia virus promoter, expressed CAT activity. No CAT activity was detected either in uninfected cells or fowlpox virus-infected cells. These results indicated that a heterologous vaccinia virus promoter can regulate expression of a foreign gene in fowlpox virus.
通过瞬时表达试验评估了痘苗病毒启动子对禽痘病毒中外源基因的调控作用。用含有连接到痘苗病毒启动子上的氯霉素乙酰转移酶(CAT)基因的质粒DNA转染感染了禽痘病毒的鹌鹑细胞,这些细胞表达了CAT活性。在未感染的细胞或感染了禽痘病毒的细胞中均未检测到CAT活性。这些结果表明,异源痘苗病毒启动子可以调控禽痘病毒中外源基因的表达。