IspG（GcpE）催化反应中间体的 ENDOR 和 HYSCORE 研究。
An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis.
机构信息
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, 607 South Mathews Avenue, Urbana, Illinois 61801, USA.
出版信息
J Am Chem Soc. 2011 Jun 8;133(22):8400-3. doi: 10.1021/ja200763a. Epub 2011 May 16.
Abstract
IspG is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron-nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand.
摘要
IspG 是一种含有 4 个铁原子和 4 个硫原子的蛋白，在异戊烯基生物合成中执行一个重要的还原步骤。通过对标记样品进行电子-核双共振（ENDOR）和超精细亚层相关（HYSCORE）光谱分析，我们专门对反应中间体中的超精细相互作用进行了分配。这些结果有助于阐明反应中间体的性质，支持簇中独特的第四个铁原子与配体的 C2 和 O3 之间的直接相互作用。
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