Berkeley Sensor and Actuator Center, Department of Mechanical Engineering, University of California at Berkeley, Berkeley California 94720, United States.
ACS Nano. 2011 Jun 28;5(6):5067-71. doi: 10.1021/nn201142f. Epub 2011 May 18.
The local temperature response inside single living cells upon external chemical and physical stimuli was characterized using quantum dots as nano thermometers. The photoluminescence spectral shifts from endocytosed quantum dots were used to map intracellular heat generation in NIH/3T3 cells following Ca(2+) stress and cold shock. The direct observation of inhomogeneous intracellular temperature progression raises interesting new possibilities, including further innovations in nanomaterials for sensing local responses, as well as the concept of subcellular temperature gradient for signaling and regulation in cells.
利用量子点作为纳米温度计，研究了单个活细胞在外部化学和物理刺激下的局部温度响应。通过内吞量子点的光致发光光谱位移，映射了 NIH/3T3 细胞在 Ca(2+)应激和冷休克后的细胞内热生成。对不均匀的细胞内温度变化的直接观察提出了有趣的新可能性，包括用于感应局部响应的纳米材料的进一步创新，以及细胞内信号和调节的亚细胞温度梯度的概念。
