Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Oman.
Ren Fail. 2011;33(5):518-23. doi: 10.3109/0886022X.2011.577546.
Generation of reactive oxygen species (ROS) is involved in the nephrotoxicity of platinum anticancer drugs. This study involved incubation of human embryonic kidney (HEK) 293 cells in cell culture media supplemented with cisplatin or oxaliplatin in the presence or absence of curcumin, a well-studied antioxidant. Thereafter several indices of oxidative stress have been measured, which included glutathione (GSH), total antioxidant capacity (TAC), and antioxidant enzymes [(superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidases (GPX)]. The impact of platinum drugs on cells viability, lipid peroxidation, and lactate dehydrogenase leakage was also examined. The results show that at both acute (60 min) and chronic (24 h) durations of incubation, cisplatin and oxaliplatin induced oxidative stress as evidenced by significant inhibition of the activities of SOD, CAT, and GPX enzymes as well as significant reduction of the concentrations of GSH and TAC. Curcumin ameliorated the oxidative stress induced by these insults by significantly restoring the measured oxidative indices. Our findings provide evidence that curcumin significantly ameliorates oxidative stress induced by both cisplatin and oxaliplatin in HEK cells.
活性氧（ROS）的产生与铂类抗癌药物的肾毒性有关。本研究将人胚肾（HEK）293 细胞在含有顺铂或奥沙利铂的细胞培养基中孵育，同时存在或不存在姜黄素，这是一种研究充分的抗氧化剂。然后测量了几种氧化应激指标，包括谷胱甘肽（GSH）、总抗氧化能力（TAC）和抗氧化酶[（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）]。还检查了铂类药物对细胞活力、脂质过氧化和乳酸脱氢酶漏出的影响。结果表明，在急性（60 分钟）和慢性（24 小时）孵育期间，顺铂和奥沙利铂均诱导氧化应激，这表现为 SOD、CAT 和 GPX 酶的活性显著抑制以及 GSH 和 TAC 的浓度显著降低。姜黄素通过显著恢复所测量的氧化指标，改善了这些损伤引起的氧化应激。我们的研究结果提供了证据，表明姜黄素显著改善了 HEK 细胞中顺铂和奥沙利铂引起的氧化应激。
