Mengulluoglu Umut, Altug Cagri, Ertugrul H Deniz, Yildiz Abdulkerim, Ekici E Melis, Dinckaya Erhan
Ege University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Turkey.
Artif Cells Blood Substit Immobil Biotechnol. 2012 Feb;40(1-2):97-102. doi: 10.3109/10731199.2011.597757. Epub 2011 Aug 12.
A low-cost and sensitive amperometric biosensor was developed for the determination of α-amylase activity. The biosensor was constructed by immobilizing glucose oxidase-gelatin via glutaraldehyde on the Au electrode surface. Measurements were carried out chronoamperometrically at -0.7 V. Several parameters such as glucose oxidase activity, gelatin amount, and glutaraldehyde percentage for cross-linking were optimized. Optimum pH, optimum temperature, repeatability, and storage stabilities of the biosensor were identified. Under the optimum experimental conditions, a linear calibration curve was obtained for α-amylase between 0.819 and 13.110 U/ml. Sample analyses were carried out by detecting α-amylase activities in baker's yeast samples.
开发了一种用于测定α-淀粉酶活性的低成本、高灵敏度的安培型生物传感器。该生物传感器是通过戊二醛将葡萄糖氧化酶-明胶固定在金电极表面构建而成的。在-0.7 V下采用计时电流法进行测量。对葡萄糖氧化酶活性、明胶用量和交联用戊二醛百分比等几个参数进行了优化。确定了生物传感器的最佳pH值、最佳温度、重复性和储存稳定性。在最佳实验条件下，获得了α-淀粉酶在0.819至13.110 U/ml之间的线性校准曲线。通过检测面包酵母样品中的α-淀粉酶活性进行样品分析。
