Neurology Department, University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, OH 44106–5040, USA.
Epilepsy Behav. 2011 Jul;21(3):267-70. doi: 10.1016/j.yebeh.2011.04.006. Epub 2011 May 14.
Longitudinal hippocampal pathways are needed for seizure synchronization, and there is evidence that their transection may abolish seizures. However, the effect of such transection on memory is unknown. In this study, we investigated the effect of transverse CA3 transections on memory function in Sprague-Dawley rats. With a stereotactic knife, a single CA3 transection was made unilaterally (n=5) or bilaterally (n=5). Sham surgery was done in another group (n=4). Morris water maze and novel object recognition tests were started 18 days later and revealed no significant differences between transected animals and controls. Cresyl-violet brain staining confirmed the locations of transections in the CA3 region. We conclude that normal performances in Morris water maze and novel object recognition tests do not appear to require intact transmission throughout the whole length of CA3, supporting the hypothesis that CA3 transections may be used in temporal lobe epilepsy to interrupt seizure circuitry while preserving memory.
长程海马通路上行对于癫痫发作的同步化是必需的，有证据表明其切断可能消除癫痫发作。然而，这种切断对记忆的影响尚不清楚。在这项研究中，我们研究了横向 CA3 切断对 Sprague-Dawley 大鼠记忆功能的影响。使用立体定向刀，在单侧（n=5）或双侧（n=5）进行单次 CA3 切断。另一组（n=4）进行假手术。18 天后开始进行 Morris 水迷宫和新物体识别测试，未发现切断动物与对照组之间存在显著差异。亚甲蓝脑染色证实了 CA3 区域的切断位置。我们得出结论，在 Morris 水迷宫和新物体识别测试中的正常表现似乎并不需要 CA3 全长的完整传递，这支持了 CA3 切断术可用于颞叶癫痫以中断发作电路而保留记忆的假说。
