Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Microbes Environ. 2010;25(1):45-8. doi: 10.1264/jsme2.me09160.
We analyzed the quantity and diversity of nitrite reductase genes (nirS and nirK) in rice paddy soil before and after inducing denitrification. A quantitative PCR analysis showed that the copy number of nirK, but not nirS, increased significantly in response to the denitrification-inducing conditions. Diverse nirS and nirK clones were identified by clone library analyses. Clones related to the NirS of Burkholderiales and Rhodocyclales, and NirK distantly related to known denitrifiers increased their proportion in response to the denitrification-inducing conditions, and therefore, might be involved in denitrification in rice paddy soil.
我们分析了诱导反硝化前后稻田土壤中亚硝酸盐还原酶基因（nirS 和 nirK）的数量和多样性。定量 PCR 分析表明，nirK 的拷贝数而非 nirS 的拷贝数在响应反硝化诱导条件时显著增加。通过克隆文库分析鉴定了多样的 nirS 和 nirK 克隆。与伯克霍尔德氏菌纲和红环菌纲的 NirS 以及与已知反硝化菌关系较远的 NirK 克隆的比例在响应反硝化诱导条件时增加，因此可能参与了稻田土壤中的反硝化作用。
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