体内标记的晚期猿猴病毒40 RNA的转录模式：在mRNA 3'末端之外等摩尔转录。
Transcription pattern of in vivo-labeled late simian virus 40 RNA: equimolar transcription beyond the mRNA 3' terminus.
作者信息
Ford J P, Hsu M T
出版信息
J Virol. 1978 Dec;28(3):795-801. doi: 10.1128/JVI.28.3.795-801.1978.
Abstract
We have examined the pattern of RNA transcription on the L (late) DNA strand late in the simian virus 40 virus infectious cycle by separating pulse-labeled RNA chains according to size and hybridizing to an ordered series of DNA fragments obtained by restriction enzyme digestion. From this analysis, the 5' end of the nascent transcript occurs a short distance counterclockwise from 0.735. Transcription proceeds in a clockwise direction. There is equimolar transcription for at least 1,000 nucleotides beyond the 3' end of the mRNA (0.175). The 3' terminus for the largest molecules is in 0.43--0.655. Molecules which represent more than one circuit of the genome are not more than 1% of the total population of nascent molecules. Implications of these findings for models of the biogenesis of mRNA are discussed.
摘要
我们通过根据大小分离脉冲标记的RNA链，并与通过限制性酶切获得的一系列有序DNA片段杂交，研究了猿猴病毒40病毒感染周期后期L（晚期）DNA链上的RNA转录模式。通过该分析，新生转录本的5'端在距0.735逆时针方向短距离处出现。转录按顺时针方向进行。在mRNA（0.175）的3'端之外至少1000个核苷酸处存在等摩尔转录。最大分子的3'末端位于0.43 - 0.655处。代表基因组多个循环的分子不超过新生分子总数的1%。讨论了这些发现对mRNA生物发生模型的意义。
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