多瘤病毒基因组的转录：巨型晚期多瘤病毒特异性RNA的合成与切割
Transcription of the polyoma virus genome: synthesis and cleavage of giant late polyoma-specific RNA.
作者信息
Acheson N H, Buetti E, Scherrer K, Weil R
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2231-5. doi: 10.1073/pnas.68.9.2231.
Abstract
The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized "late" polyoma-specific RNA appears as "giant" molecules of heterogeneous size, up to several times larger than a strand of polyoma DNA (1.5 x 10(6) daltons). Treatment with dimethylsulfoxide or urea showed that the large size of these molecules is not due to aggregation. Giant polyoma-specific RNA is strikingly similar in size distribution to "nuclear messenger-like" RNA ("heterogeneous nuclear" RNA) of the host cell. Subsequent to its synthesis, some of the giant polyoma-specific RNA appears to be cleaved to at least three smaller species.
摘要
通过对感染多瘤病毒的培养小鼠肾细胞中提取的RNA进行电泳和沉降分析，估算了病毒特异性RNA的大小。新合成的“晚期”多瘤病毒特异性RNA呈现为大小不均一的“巨大”分子，其大小比多瘤病毒DNA链（1.5×10⁶道尔顿）大几倍。用二甲基亚砜或尿素处理表明，这些分子的大尺寸并非由于聚集所致。巨大的多瘤病毒特异性RNA在大小分布上与宿主细胞的“核信使样”RNA（“不均一核”RNA）惊人地相似。在其合成之后，一些巨大的多瘤病毒特异性RNA似乎被切割成至少三种较小的种类。
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