未整合的禽RNA肿瘤病毒双链DNA中间体的分析
Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates.
作者信息
Hsu T W, Sabran J L, Mark G E, Guntaka R V, Taylor J M
出版信息
J Virol. 1978 Dec;28(3):810-8. doi: 10.1128/JVI.28.3.810-818.1978.
Abstract
Previous studies by Guntaka et al. have shown that the unintegrated DNA intermediates of avian RNA tumor virus replication can be readily isolated from cultures of the quail tumor line QT-6 at 1 day after infection. The intermediates include double-stranded linear and covalently closed circular DNA species. Using the analysis procedure of Southern together with previously obtained information regarding the sites of action of certain restriction endonucleases on avian sarcoma virus DNA, we have further characterized the viral DNA intermediates. Evidence is presented that, relative to the RNA genome, most of the linear species possess a direct terminal sequence redundancy equivalent to 0.5 X 10(6) +/- 0.3 X 10(6) daltons of double-stranded DNA. Some of the circular forms also possess a sequence redundancy of 0.21 X 10(6) +/- 0.03 X 10(6) daltons.
摘要
贡塔卡等人之前的研究表明，禽RNA肿瘤病毒复制过程中的未整合DNA中间体在感染后1天，可很容易地从鹌鹑肿瘤细胞系QT - 6的培养物中分离出来。这些中间体包括双链线性和共价闭合环状DNA种类。利用Southern分析程序以及先前获得的关于某些限制性内切酶对禽肉瘤病毒DNA作用位点的信息，我们进一步对病毒DNA中间体进行了表征。有证据表明，相对于RNA基因组，大多数线性种类具有相当于0.5×10⁶±0.3×10⁶道尔顿双链DNA的直接末端序列冗余。一些环状形式也具有0.21×10⁶±0.03×10⁶道尔顿的序列冗余。
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