禽肉瘤病毒感染细胞胞质中的病毒特异性DNA是细胞核中共价闭合环状病毒DNA的前体。
Virus-specific DNA in the cytoplasm of avian sarcoma virus-infected cells is a precursor to covalently closed circular viral DNA in the nucleus.
作者信息
Shank P R, Varmus H E
出版信息
J Virol. 1978 Jan;25(1):104-4. doi: 10.1128/JVI.25.1.104-104.1978.
Abstract
Three principal forms of viral DNA have been identified in cells infected with avian sarcoma virus: (i) a linear duplex molecule synthesized in the cytoplasm, (ii) a covalently closed circular molecule found in the nucleus, and (iii) proviral DNA covalently linked to high-molecular-weight cell DNA. To define precursor product relationships among these forms of viral DNA, we performed pulsechase experiments using 5-bromodeoxyuridine to label by density the linear species of viral DNA in the cytoplasm during the first 4 h after infection. After a 4-to 8-h chase with thymidine, a portion of the density-labeled viral DNA was transported to the nucleus and converted to a covalently closed circular form. We conclude that linear viral DNA, synthesized in the cytoplasm, is the precursor to closed circular DNA observed in the nucleus.
摘要
在感染禽肉瘤病毒的细胞中已鉴定出三种主要形式的病毒DNA：（i）在细胞质中合成的线性双链分子，（ii）在细胞核中发现的共价闭合环状分子，以及（iii）与高分子量细胞DNA共价连接的前病毒DNA。为了确定这些形式的病毒DNA之间的前体-产物关系，我们进行了脉冲追踪实验，在感染后的前4小时内使用5-溴脱氧尿苷通过密度标记细胞质中的线性病毒DNA。在用胸苷进行4至8小时的追踪后，一部分密度标记的病毒DNA被转运到细胞核并转化为共价闭合环状形式。我们得出结论，在细胞质中合成的线性病毒DNA是在细胞核中观察到的闭合环状DNA的前体。
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