Naeem K, Caywood D D, Werdin R E, Goyal S M
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, St Paul 55108.
Am J Vet Res. 1990 Apr;51(4):640-4.
A field strain (87-8363) of bovid herpesvirus-4 (BHV-4) isolated from an aborted bovine fetus was used to inoculate pregnant rabbits. Eleven rabbits in midgestation were alloted to 4 groups consisting of 3 infected groups and 1 control group. Rabbits were inoculated with BHV-4 or mock-infected cell culture preparations via IV, intravaginal, and intrauterine routes. Mild vulvovaginitis and endometritis were observed after intravaginal and IV inoculation of BHV-4, whereas intrauterine inoculation of BHV-4 resulted in abortion of hemorrhagic fetuses and nonsuppurative endometritis. Virus was successfully isolated from organ explants of fetal tissues. Rabbits seroconverted 1 week after infection as detected by results of an indirect immunofluorescence assay.
从一头流产牛胎儿中分离出的牛疱疹病毒4型（BHV - 4）的田间毒株（87 - 8363）用于接种怀孕兔子。将11只妊娠中期的兔子分为4组，包括3个感染组和1个对照组。通过静脉内、阴道内和子宫内途径，给兔子接种BHV - 4或模拟感染的细胞培养制剂。阴道内和静脉内接种BHV - 4后观察到轻度外阴阴道炎和子宫内膜炎，而子宫内接种BHV - 4导致出血性胎儿流产和非化脓性子宫内膜炎。病毒成功地从胎儿组织的器官外植体中分离出来。通过间接免疫荧光试验结果检测，兔子在感染后1周血清转化。
