Abramson A L, DiLorenzo T P, Steinberg B M
Department of Otolaryngology and Communicative Disorders, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Arch Otolaryngol Head Neck Surg. 1990 May;116(5):604-7. doi: 10.1001/archotol.1990.01870050104017.
The carbon dioxide laser is widely used for the vaporization of lesions in patients with laryngeal papillomatosis. In this study, the smoke plume resulting from the laser treatment of laryngeal papillomas was analyzed for the presence of human papillomavirus DNA. Plumes were collected with a suction tip and trapped in phosphate-buffered saline. The aspirates were then analyzed for the presence of human papillomavirus DNA by Southern blot hybridization. Human papillomavirus DNA cannot be detected in the smoke plume from vaporization of laryngeal papillomas unless direct suction contact is made with the papilloma tissue during surgery. The implications of these findings are discussed.
二氧化碳激光广泛应用于喉乳头状瘤病患者病变组织的汽化治疗。在本研究中，对激光治疗喉乳头状瘤产生的烟雾进行分析，以检测人乳头瘤病毒DNA的存在。用吸头收集烟雾并捕获于磷酸盐缓冲盐水中。然后通过Southern印迹杂交分析吸出物中人乳头瘤病毒DNA的存在情况。除非在手术过程中直接对乳头瘤组织进行抽吸接触，否则在喉乳头状瘤汽化产生的烟雾中无法检测到人乳头瘤病毒DNA。本文讨论了这些发现的意义。
