Leung-Tack J, Tavera C, Gensac M C, Martinez J, Colle A
INSERM U 133, Faculté de Médecine, Toulouse, France.
Exp Cell Res. 1990 May;188(1):16-22. doi: 10.1016/0014-4827(90)90272-c.
Cystatin C, a cysteine protease inhibitor, has recently been suggested to be a potent regulator in inflammatory processes. Human cystatin C was isolated from the urine of one patient suffering from tubular disorders and was tested for its effects on two functions of human polymorphonuclear neutrophils (PMN): O2- release and phagocytosis. Slow-form or (des 1-4) cystatin C and fast-form or (des 1-8) cystatin C differed by the presence in (des 1-4) cystatin C only of the N-terminal tetrapeptide Lys-Pro-Pro-Arg. Whereas (des 1-8) cystatin C did not seem to interfere with PMN functions at physiological concentrations, (des 1-4) cystatin C induced an inhibition of PMN phagocytosis-associated respiratory burst in response to opsonized zymosan particles. The inhibition may be attributed to the tetrapeptide Lys-Pro-Pro-Arg which has been synthesized and shown to have the same inhibitor effects, at concentrations similar to those required for (des 1-4) cystatin C. These results support a potential role for cystatin C as a modulator during inflammation.
胱抑素C，一种半胱氨酸蛋白酶抑制剂，最近被认为是炎症过程中的一种有效调节剂。人胱抑素C是从一名患有肾小管疾病的患者尿液中分离出来的，并测试了其对人多形核中性粒细胞（PMN）两种功能的影响：氧自由基释放和吞噬作用。慢形式或（缺失1-4）胱抑素C与快形式或（缺失1-8）胱抑素C的区别仅在于（缺失1-4）胱抑素C中仅存在N端四肽赖氨酸-脯氨酸-脯氨酸-精氨酸。虽然（缺失1-8）胱抑素C在生理浓度下似乎不干扰PMN功能，但（缺失1-4）胱抑素C可抑制PMN对调理酵母聚糖颗粒的吞噬相关呼吸爆发。这种抑制作用可能归因于四肽赖氨酸-脯氨酸-脯氨酸-精氨酸，已合成该四肽并证明其在与（缺失1-4）胱抑素C所需浓度相似的情况下具有相同的抑制作用。这些结果支持了胱抑素C在炎症过程中作为调节剂的潜在作用。
