Division of Biological Science and Technology, Yonsei University, Wonju, 220-710, Republic of Korea.
Mol Biol Rep. 2012 Feb;39(2):1087-93. doi: 10.1007/s11033-011-0835-x. Epub 2011 May 17.
Autophagy is a membrane trafficking process involved in intracellular degradation and recycling in eukaryotic cells. DRAM2 (damage-regulated autophagy modulator 2) is a homologue of DRAM that regulates p53-mediated cell death. As its name implies, DRAM expression induces autophagy in a p53-dependent manner; however, the role of DRAM2 in autophagy is not clear. In this study, we report that DRAM2 expression contributes to autophagy induction. Overexpression of DRAM2 induces cytoplasmic GFP-LC3 punctuates, and increases the level of endogenous LC3-II. Moreover, the silencing of endogenous DRAM2 interferes with starvation-induced autophagy. Thus, we propose that DRAM2 as well as DRAM are involved in autophagy.
自噬是真核细胞中涉及细胞内降解和再循环的一种膜运输过程。DRAM2（损伤调节自噬调节剂 2）是 DRAM 的同源物，可调节 p53 介导的细胞死亡。顾名思义，DRAM 表达以 p53 依赖的方式诱导自噬；然而，DRAM2 在自噬中的作用尚不清楚。在这项研究中，我们报告了 DRAM2 表达有助于自噬的诱导。DRAM2 的过表达诱导细胞质 GFP-LC3 点状，并增加内源性 LC3-II 的水平。此外，内源性 DRAM2 的沉默干扰了饥饿诱导的自噬。因此，我们提出 DRAM2 以及 DRAM 都参与了自噬。
