Wang Jia-you, Tang Chun-zhi, He Zhen-quan, Zhang Jin, Hao Mu-feng, Ma Chun-mei, Yang Zhong-hua, Lai Xin-sheng
Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
Zhen Ci Yan Jiu. 2011 Feb;36(1):36-9.
To observe the effect of different strength of acupuncture stimulation on blood pressure and plasma endothelin (E)-1 in spontaneous hypertension rats (SHR) ,so as to seek a better acupuncture parameter for clinical treatment of hypertension.
Twenty-eight 9-week-old SHRs were randomized into mild-stimulation group, moderate-stimulation group, strong-stimulation group and model group (n = 7 in each group). Seven normotensive SD rats served as a normal control group. Acupuncture stimulation with mild, moderate and strong stimulation was applied to bilateral "Taichong" (LR 3) for 5 min, once daily for 7 days. Blood pressure (BP) was determined by using a non-invasive BP-6 detection system. Plasma ET-1 was assayed by radioimmunoassay.
Compared with the model group, the systolic pressure of the moderate-stimulation group on the 6th and 7th day was decreased significantly after acupuncture of "Taichong" (LR 3) (P<0. 01), being significantly lower than that of the mild- and strong-stimulation groups (P<0. 01). In comparison with the normal control group, plasma ET-1 level in the model group was increased significantly (P<0.01), while compared with the model group, only that in the moderate-stimulation group was down-regulated considerably (P<0. 01). No significant differences were found between the mild-stimulation and model groups, between the strong-stimulation and model groups, and between the mild-stimulation and strong-stimulation groups in plasma ET-1 level (P>0. 05).
Moderate-stimulation of "Taichong" (LR 3) can lower blood pressure and plasma EA-1 level in spontaneous hypertension rats. The reduced level of plasma ET-1 may be one of its mechanisms underlying improving hypertension.
观察不同强度针刺刺激对自发性高血压大鼠（SHR）血压及血浆内皮素（ET）-1的影响，以寻求更适合临床治疗高血压的针刺参数。
将28只9周龄的SHR随机分为轻刺激组、中刺激组、强刺激组和模型组（每组7只）。7只血压正常的SD大鼠作为正常对照组。分别对双侧“太冲”（LR 3）进行轻、中、强刺激针刺5分钟，每日1次，共7天。采用无创BP-6检测系统测定血压（BP）。采用放射免疫分析法测定血浆ET-1。
与模型组相比，针刺“太冲”（LR 3）后，中刺激组第6天和第7天的收缩压显著降低（P<0.01），明显低于轻刺激组和强刺激组（P<0.01）。与正常对照组相比，模型组血浆ET-1水平显著升高（P<0.01），而与模型组相比，只有中刺激组血浆ET-1水平显著下调（P<0.01）。轻刺激组与模型组、强刺激组与模型组、轻刺激组与强刺激组之间血浆ET-1水平比较，差异均无统计学意义（P>0.05）。
对“太冲穴”（LR 3）进行中等强度刺激可降低自发性高血压大鼠的血压和血浆ET-1水平。血浆ET-1水平降低可能是其改善高血压的机制之一。
