[不同模拟针刺手法对自发性高血压大鼠血压及心肌血管紧张素Ⅱ含量的影响]

[Impact of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin II content in spontaneous hypertension rats].

作者信息

Yang Hua-Yuan, Zhong Xiao-Hong, Liu Tang-Yi, Kuai Le, Gao Ming

机构信息

College of Acu-moxibustion and Massage, Shanghai University of Chinese Medicine, Shanghai 201203, China.

出版信息

Zhen Ci Yan Jiu. 2008 Jun;33(3):186-90.

PMID:18807722

Abstract

OBJECTIVE

To observe the effect of different emulated acupuncture-needle manipulations on blood pressure and myocardial angiotensin (Ang) II content in spontaneously hypertensive rats (SHR), so as to seek a better acupuncture parameter for clinical treatment of hypertension.

METHODS

Thirty-three SHR were randomly divided into mild-stimulation group (n=8), moderate-stimulation group (n=8), heavy-stimulation group (n=8) and model group (n=9). Additionally, other 8 normal rats were made up of control group. Blood pressure (BP) was determined by using a non-invasive BP detection system. Myocardial Ang II content was assayed by radioimmunoassay. The emulated acupuncture-needle twirling angles and frequencies of 144 degrees, 75 r/min; 255 degrees, 111 r/min; and 360 degrees, 140 r/min in mild, moderate and heavy manipulations were applied to bilateral "Zusanli" (ST 36) and "Taichong" (LR 3) for 3 min, once daily for 3 weeks, with an interval of 2 days between every two weeks.

RESULTS

Compared with pre-therapy, BP values of both heavy-stimulation and model groups increased significantly (P<0.01); and compared with model group, BP values of mild-stimulation and moderate-stimulation groups decreased significantly after the therapy (P<0.05), but were still higher than that of control group (P<0.05). In addition, myocardial Ang II contents of mild-stimulation, moderate-stimulation, heavy-stimulation and model groups were significantly higher than that of control group (P<0.05). No significant differences were found between mild-stimulation and moderate-stimulation groups, and between heavy-stimulation and model groups after therapy in BP, and among mild-stimulation, moderate-stimulation, heavy-stimulation and model groups in myocardial Ang II contents (P>0.05).

CONCLUSION

Mild and moderate manipulations of ST36 and LR3 have apparent influence on BP, but no apparent influence on myocardial Ang II content.

摘要

目的

观察不同模拟针刺手法对自发性高血压大鼠（SHR）血压及心肌血管紧张素（Ang）Ⅱ含量的影响，以寻求更适合临床治疗高血压的针刺参数。

方法

将33只SHR随机分为轻刺激组（n = 8）、中刺激组（n = 8）、重刺激组（n = 8）和模型组（n = 9）。另外，选取8只正常大鼠作为对照组。采用无创血压检测系统测定血压（BP）。用放射免疫分析法测定心肌AngⅡ含量。轻、中、重手法分别以144度、75转/分钟；255度、111转/分钟；360度、140转/分钟的模拟针刺捻转角度和频率针刺双侧“足三里”（ST 36）和“太冲”（LR 3），留针3分钟，每日1次，共3周，每两周间隔2天。

结果

与治疗前相比，重刺激组和模型组血压值均显著升高（P < 0.01）；与模型组相比，轻刺激组和中刺激组治疗后血压值显著降低（P < 0.05），但仍高于对照组（P < 0.05）。此外，轻刺激组、中刺激组、重刺激组和模型组心肌AngⅡ含量均显著高于对照组（P < 0.05）。治疗后，轻刺激组和中刺激组之间、重刺激组和模型组之间血压无显著差异，轻刺激组、中刺激组、重刺激组和模型组之间心肌AngⅡ含量也无显著差异（P > 0.05）。