Enteric NeuroScience Program and Department of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Neurogastroenterol Motil. 2011 Aug;23(8):760-5. doi: 10.1111/j.1365-2982.2011.01729.x. Epub 2011 May 17.
Depletion of interstitial cells of Cajal (ICC) is associated with several gastrointestinal (GI) motility disorders. Changes in ICC networks are usually detected by immunolabeling for the receptor tyrosine kinase Kit. Ano1 (DOG1 or TMEM16A) was recently described as a marker of ICC in GI tract. Our aim was to determine whether Ano1 immunoreactivity can be used as a reliable marker for ICC in tissues from patients with motility disorders.
Four tissues from patients with normal ICC numbers and four tissues from patients with slow transit constipation and loss of Kit-positive ICC were studied. Interstitial cells of Cajal were detected by double labeling using antisera to Kit and Ano1.
Both the processes and cell bodies of ICC in tissue from controls and slow transit constipation were immunoreactive for Ano1. There was a near complete overlap between Kit and Ano1 immunoreactivity. Tissues from patients with slow transit constipation contained significantly fewer Ano1-positive ICC than control tissues. The numbers of ICC identified by Ano1 and Kit immunoreactivity were nearly identical across the range of ICC numbers from an average of 1.64 to 7.05 cells per field and correlated with an R(2) value of 0.99.
CONCLUSIONS & INFERENCES: Ano1 is a reliable and sensitive marker for detecting changes in ICC networks in humans. Labeling with antibodies selective for Ano1 reproducibly detects depletion of Kit-positive ICC in tissues from patients with slow transit constipation.
Cajal 间质细胞（ICC）耗竭与多种胃肠道（GI）运动障碍有关。ICC 网络的变化通常通过免疫标记受体酪氨酸激酶 Kit 来检测。Ano1（DOG1 或 TMEM16A）最近被描述为 GI 道 ICC 的标志物。我们的目的是确定 Ano1 免疫反应是否可作为运动障碍患者组织中 ICC 的可靠标志物。
研究了 4 例 ICC 数量正常的患者组织和 4 例慢传输性便秘且 Kit 阳性 ICC 缺失的患者组织。使用针对 Kit 和 Ano1 的抗血清进行双重标记来检测 Cajal 间质细胞。
对照组和慢传输性便秘患者的 ICC 过程和细胞体均对 Ano1 呈免疫反应性。Kit 和 Ano1 免疫反应性之间几乎完全重叠。与对照组织相比，慢传输性便秘患者的 Ano1 阳性 ICC 数量明显减少。通过 Ano1 和 Kit 免疫反应性鉴定的 ICC 数量在 ICC 数量范围内几乎相同，平均每视野为 1.64 到 7.05 个细胞，与 R² 值为 0.99 相关。
Ano1 是检测人类 ICC 网络变化的可靠和敏感标志物。针对 Ano1 的抗体标记可重复性地检测到慢传输性便秘患者组织中 Kit 阳性 ICC 的耗竭。
