永久性遗传资源：佛罗里达海牛（Trichechus manatus latirostris）濒危种的 18 个新的多态性微卫星标记。

PERMANENT GENETIC RESOURCES: Eighteen new polymorphic microsatellite markers for the endangered Florida manatee, Trichechus manatus latirostris.

机构信息

Florida Fish and Wildlife Research Institute, 100 Eighth Avenue S.E. Saint Petersburg, FL 33701-5095, USA, Mote Marine Laboratory, 1600 Thompson Parkway, Sarasota, FL 34236-1004, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):328-31. doi: 10.1111/j.1471-8286.2007.01944.x.

DOI:10.1111/j.1471-8286.2007.01944.x

PMID:21585782

Abstract

Here we describe 18 polymorphic microsatellite loci for Trichechus manatus latirostris (Florida manatee), isolated using a polymerase chain reaction-based technique. The number of alleles at each locus ranged from two to four (mean = 2.5) in specimens from southwest (n = 58) and northeast (n = 58) Florida. Expected and observed heterozygosities ranged from 0.11 to 0.67 (mean = 0.35) and from 0.02 to 0.78 (mean = 0.34), respectively. Departures from Hardy-Weinberg equilibrium occurred at two loci. There was no evidence of genotypic disequilibrium for any pair of loci. For individual identification, mean random-mating and θ-corrected match probabilities were 9.36 × 10(-7) and 1.95 × 10(-6) , respectively.

摘要

我们在这里描述了 18 个用于佛罗里达海牛（Trichechus manatus latirostris）的多态微卫星位点，这些位点是使用聚合酶链反应（PCR）技术分离出来的。在来自佛罗里达州西南部（n=58）和东北部（n=58）的样本中，每个位点的等位基因数从两个到四个不等（平均值为 2.5）。预期和观察到的杂合度分别在 0.11 到 0.67 之间（平均值为 0.35）和 0.02 到 0.78 之间（平均值为 0.34）。两个位点偏离了Hardy-Weinberg 平衡。任何两个位点之间都没有基因型不平衡的证据。对于个体识别，平均随机交配和θ校正匹配概率分别为 9.36×10(-7)和 1.95×10(-6)。