永久性遗传资源：12 个多态性微卫星标记用于骨鱼，Albula vulpes 和两个近缘种。

PERMANENT GENETIC RESOURCES: Twelve polymorphic microsatellite markers for the bonefish, Albula vulpes and two congeners.

机构信息

Florida Fish and Wildlife Research Institute, 100 Eighth Avenue S.E. Saint Petersburg, FL 33701-5095, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):354-6. doi: 10.1111/j.1471-8286.2007.01954.x.

DOI:10.1111/j.1471-8286.2007.01954.x

Abstract

Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8.8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0.07 to 0.77 (mean = 0.42) and from 0.07 to 0.84 (mean = 0.48), respectively. There were no significant departures from Hardy-Weinberg equilibrium and no evidence of genotypic disequilibrium between any pair of loci. In a cross-amplification test, all markers yielded appropriately sized alleles for specimens of the provisional Albula sp. B and 11 of the 12 loci amplified for those of Albula glossodonta.

摘要

利用聚合酶链式反应（PCR）技术，从鲈形目隆头鱼科的波纹唇鱼（Albula vulpes）中分离出 12 个微卫星多态性位点。在佛罗里达州南部的 37 个样本中，等位基因的数量从 2 到 23 不等（平均值为 8.8）。观察到的和预期的杂合度分别为 0.07 至 0.77（平均值为 0.42）和 0.07 至 0.84（平均值为 0.48）。没有明显偏离哈迪-温伯格平衡，也没有任何一对位点之间存在基因型不平衡的证据。在交叉扩增测试中，所有标记都为暂定波纹唇鱼（Albula sp. B）和 11 个黄唇鱼（Albula glossodonta）的样本产生了适当大小的等位基因。