开发 15 个多态性微卫星标记用于大西洋马鲛（Megalops atlanticus）的捕获-再捕获研究。

Development of 15 polymorphic microsatellite markers in the Atlantic tarpon (Megalops atlanticus) for capture-recapture studies.

机构信息

Florida Fish and Wildlife Research Institute, 100 Eighth Avenue SE, Saint Petersburg, Florida 33701-5095, USA.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):126-8. doi: 10.1111/j.1471-8286.2007.01924.x.

DOI:10.1111/j.1471-8286.2007.01924.x

Abstract

Fifteen polymorphic microsatellite DNA loci for Atlantic tarpon, Megalops atlanticus, were isolated by using PIMA, a polymerase chain reaction-based technique. The number of alleles at each locus ranged from two to 24 (mean = 7.7) in 65 specimens from Tampa Bay, Florida. Observed and expected heterozygosities ranged from 0.27 to 0.92 (mean = 0.60) and from 0.28 to 0.95 (mean = 0.62), respectively. Genotypes at one locus deviated significantly from Hardy-Weinberg equilibrium. In exact tests for genotypic disequilibrium, there was no evidence of associations between any pair of loci. Overall, loci were well resolved and highly polymorphic, confirming their suitability for DNA fingerprinting applications and other genetic studies.

摘要

15 个多态微卫星 DNA 位点通过 PIMA（基于聚合酶链反应的技术）从大西洋马鲛鱼（Megalops atlanticus）中分离出来。在来自佛罗里达州坦帕湾的 65 个样本中，每个位点的等位基因数从 2 到 24 不等（平均值为 7.7）。观察到的和预期的杂合度分别从 0.27 到 0.92（平均值为 0.60）和从 0.28 到 0.95（平均值为 0.62）不等。一个位点的基因型与哈迪-温伯格平衡显著偏离。在确切的基因型不平衡检验中，没有证据表明任何两个位点之间存在关联。总体而言，这些位点分辨率良好且高度多态，证实了它们适用于 DNA 指纹图谱应用和其他遗传研究。