永久遗传资源：用于巴西利什曼原虫遗传研究的 12 个微卫星基因座。
PERMANENT GENETIC RESOURCES: A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis.
机构信息
Genetique et Evolution des Maladies Infectieuses, IRD/CNRS (UMR2724), Centre, IRD, 911, Avenue Agropolis BP 64501, F-34394, Montpellier Cedex 5, France, Instituto de Medicina Tropical 'Alexander von Humboldt', Lima, Peru.
出版信息
Mol Ecol Resour. 2008 Mar;8(2):351-3. doi: 10.1111/j.1471-8286.2007.01953.x.
Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed (F(IS) = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis.
检测了 12 个巴西利什曼原虫的微卫星位点，其中 9 个是在此项工作中开发的。用这些微卫星位点对 56 株巴西利什曼原虫进行了基因分型。所研究的 12 个位点具有多态性，等位基因数从 5 到 19 不等，平均值为 9.7±4.1，观察到的杂合度平均值为 0.425±0.202。我们观察到的重要杂合子缺失（F(IS) = 0.41，P 值 = 0.004）与预期的克隆二倍体中的杂合子过剩似乎不一致。这一结果可能会重新引发关于利什曼原虫的克隆性/有性生殖的争论。这项工作验证了这些微卫星用于种群遗传学分析的潜力。
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