14 个中国药用植物箭叶淫羊藿微卫星位点及其在其他药用物种中的跨物种应用。

Fourteen microsatellite loci for the Chinese medicinal plant Epimedium sagittatum and cross-species application in other medicinal species.

机构信息

Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, China.

出版信息

Mol Ecol Resour. 2008 May;8(3):640-2. doi: 10.1111/j.1471-8286.2007.02029.x.

DOI:10.1111/j.1471-8286.2007.02029.x

Abstract

Epimedium sagittatum is a well-known medicinal plant as well as potential ground cover and ornamental plant. A total of 14 polymorphic microsatellite markers were developed for use in taxonomy, phylogenetics and conservation genetics. Markers were presented between two and eight alleles per locus. The observed heterozygosity (H(O) ) and expected heterozygosity (H(E) ) ranged from 0.03 to 0.81 and from 0.05 to 0.81, respectively. Cross-amplification among other four medicinal species revealed promising results in three to six polymorphic loci. These microsatellite loci contributed to the limited number of useful markers currently available for E. sagittatum.

摘要

箭叶淫羊藿是一种著名的药用植物，也是一种有潜力的地被植物和观赏植物。本研究共开发了 14 个多态性微卫星标记，用于分类学、系统发育学和保护遗传学。每个位点的等位基因数为 2-8 个，观测杂合度（H(O) ）和期望杂合度（H(E) ）范围分别为 0.03-0.81 和 0.05-0.81。在其他四个药用物种中的交叉扩增在三到六个多态性位点中产生了有希望的结果。这些微卫星标记为目前有限的可用标记提供了有用的补充。