旋木雀属（Remiz pendulinus）中多态性微卫星 DNA 标记

Polymorphic microsatellite DNA markers in the penduline tit, Remiz pendulinus.

机构信息

Department of Biology, Faculty of Medicine, University of Szeged, Hungary, Ecogenics GmbH, Zurich-Schlieren, Switzerland, Animal Ecology Group, Centre for Ecological and Evolutionary Studies, University of Groningen, The Netherlands.

出版信息

Mol Ecol Resour. 2008 May;8(3):692-4. doi: 10.1111/j.1471-8286.2007.02050.x.

DOI:10.1111/j.1471-8286.2007.02050.x

PMID:21585873

Abstract

To describe the exceptional mating system of the penduline tit, Remiz pendulinus, we aim to combine field observation records with DNA analysis based on polymorphic microsatellite DNA markers. Here we describe features of nine loci and their corresponding polymerase chain reaction primers. The observed number of alleles varied from four to seven and the observed heterozygosity ranged from 0.419 to 0.802. Neither of the loci is sex-linked and as linkage disequilibrium analysis showed they assort independently. Seven of the nine loci were polymorphic in the Cape penduline tit, Anthoscopus minutus.

摘要

为了描述悬垂凤头鹀（Remiz pendulinus）的特殊交配系统，我们旨在结合基于多态微卫星 DNA 标记的野外观察记录和 DNA 分析。在这里，我们描述了九个位点的特征及其相应的聚合酶链反应引物。观察到的等位基因数从四个到七个不等，观察到的杂合度从 0.419 到 0.802 不等。没有一个位点是性连锁的，连锁不平衡分析表明它们是独立分配的。九个位点中有七个在海角悬垂凤头鹀（Anthoscopus minutus）中是多态的。