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从食菌果蝇 Drosophila innubila 中分离和鉴定 30 个多态性微卫星位点。

Isolation and characterization of 30 polymorphic microsatellite loci from the mycophagous fly Drosophila innubila.

机构信息

Department of Biology, University of Rochester, Rochester, NY 14627, USA, Department of Genetics, University of Georgia, Athens, GA 30602, USA.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):939-42. doi: 10.1111/j.1755-0998.2008.02124.x.

DOI:10.1111/j.1755-0998.2008.02124.x
PMID:21585936
Abstract

Drosophila innubila is a mushroom-feeding member of the quinaria group, found in the woodlands and forests of the 'sky islands' in Arizona and New Mexico and extending south into central Mexico. Here, we describe and characterize 30 polymorphic microsatellite loci from D. innubila collected in the Chiricahua Mountains in Arizona. The number of alleles ranged from three to 21, and observed heterozygosity ranged from 0.0513 to 0.9737. Six loci were putatively X-linked, six departed from Hardy-Weinberg equilibrium, seven had evidence of null alleles, and six showed evidence of linkage disequilibrium. These markers will be useful for examining population structure of D. innubila and its association with male-killing Wolbachia.

摘要

暗黄果蝇是 quinaria 组中的一种蘑菇食性成员,分布于亚利桑那州和新墨西哥州的“天空岛屿”的林地和森林中,并向南延伸到墨西哥中部。在这里,我们描述并表征了在亚利桑那州奇里卡瓦山脉采集的暗黄果蝇的 30 个多态性微卫星位点。等位基因数从 3 到 21 不等,观察到的杂合度从 0.0513 到 0.9737 不等。6 个位点被推测为 X 连锁,6 个偏离哈迪-温伯格平衡,7 个有无效等位基因的证据,6 个有连锁不平衡的证据。这些标记将有助于研究暗黄果蝇的种群结构及其与雄性致死沃尔巴克氏体的关联。

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