永久性遗传资源：在神秘的东非鸟类——斑点喉（Modulatrix stictigula）中分离和鉴定 10 个四核苷酸微卫星位点。

PERMANENT GENETIC RESOURCES: Isolation and characterization of 10 tetranucleotide microsatellite loci in an enigmatic East African bird, the spot-throat (Modulatrix stictigula).

机构信息

Museum of Vertebrate Zoology and Department of Integrative Biology, 3101 Valley Life Science Building, University of California, Berkeley, CA 94720, USA, Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S Lake Shore Drive, Chicago, IL 60605, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):342-4. doi: 10.1111/j.1471-8286.2007.01949.x.

DOI:10.1111/j.1471-8286.2007.01949.x

PMID:21585786

Abstract

We describe the isolation of 10 tetranucleotide microsatellites from the spot-throat using an enrichment protocol. All loci were highly variable with the number of alleles ranging from six to 20 and observed heterozygosity ranging from 0.410 to 0.940. Although all loci were in Hardy-Weinberg equilibrium, locus Mst95 showed significant homozygote excess in both surveyed populations, possibly a consequence of the presence of null alleles at this locus. These loci will be used to determine the extent to which spot-throat populations are isolated in order to help set conservation priorities for this ancient African lineage.

摘要

我们描述了一种富集法从斑点喉中分离出 10 个四核苷酸微卫星。所有的位点都高度可变，等位基因数从 6 到 20 不等，观察到的杂合度从 0.410 到 0.940 不等。尽管所有的位点都处于哈迪-温伯格平衡状态，但 Mst95 位点在两个调查的种群中都表现出显著的纯合子过剩，这可能是由于该位点存在无效等位基因的结果。这些位点将用于确定斑点喉种群的隔离程度，以帮助确定这个古老的非洲谱系的保护优先级。