Department of Wildlife Ecology and Management, University Freiburg, Tennenbacher Str. 4, D-79106 Freiburg, Germany, Department of Evolutionary Biology and Animal Ecology, Faculty of Biology, University Freiburg, Hauptstrasse 1, D-79108 Freiburg, Germany, Estación Biológica de Doñana (CSIC), Pabellón del Perú, Avda. Maria Luisa s/n, 41013 Sevilla, Spain.
Mol Ecol Resour. 2008 Sep;8(5):1108-10. doi: 10.1111/j.1755-0998.2008.02171.x. Epub 2008 Jul 18.
We isolated 10 polymorphic microsatellite DNA loci from the blackcap (Sylvia atricapilla) and optimized them for future studies of population differentiation in populations with different migration strategies in southwestern Germany. The loci were screened for polymorphism using 178 individuals from two populations in Germany and Spain. The primers amplified highly variable loci characterized by two to 19 alleles per locus and their observed and expected heterozygosities range from 0.47 to 0.81 and from 0.50 to 0.91, respectively.
我们从黑头鳾（Sylvia atricapilla）中分离出 10 个多态微卫星 DNA 位点，并对其进行了优化，以便未来研究德国西南部具有不同迁徙策略的种群的种群分化。我们使用来自德国和西班牙的两个种群的 178 个个体对这些位点进行了多态性筛选。引物扩增了高度可变的位点，每个位点有 2 到 19 个等位基因，其观察到的和预期的杂合度分别在 0.47 到 0.81 之间和 0.50 到 0.91 之间。
