热带壁虎（Hemidactylus mabouia）12 个多态微卫星标记的分离与特征。

Isolation and characterization of 12 polymorphic microsatellite markers in the tropical house gecko (Hemidactylus mabouia).

机构信息

Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221-0006, USA.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1319-21. doi: 10.1111/j.1755-0998.2008.02270.x. Epub 2008 Aug 26.

DOI:10.1111/j.1755-0998.2008.02270.x

Abstract

We present 12 variable microsatellite loci isolated from the invasive tropical house gecko, Hemidactylus mabouia. Polymerase chain reaction (PCR) primers were tested on 39 individuals from two locations in Miami, Florida. Heterozygote deficiency was detected for four loci, and we attribute this to possible null alleles or population substructure. Some loci successfully amplified PCR products in several congeners, indicating their potential for use in other geckos.

摘要

我们从入侵性热带家壁虎 Hemidactylus mabouia 中分离出了 12 个可变微卫星位点。在佛罗里达州迈阿密的两个地点的 39 个人个体上测试了聚合酶链反应（PCR）引物。四个位点检测到杂合子缺失，我们认为这可能是由于存在无效等位基因或种群亚结构。一些位点在几个近缘种中成功扩增了 PCR 产物，表明它们在其他壁虎中的潜在应用。